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KMID : 0362420110490040333
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Journal of Korean Academy of Prosthodontics
2011 Volume.49 No. 4 p.333 ~ p.340
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The effect of different crystallization temperature of the hydroxyapatite coating produced
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Bae Ah-Ran
Won Hyun-Du
Lee Sung-Bok
Kim Hyeong-Seob
Woo Yi-Hyung
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Abstract
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Purpose: differentiation of human osteosarcoma cells.
Materials and Methods : Surface roughness of the titanium disks increased by anodizing treatment and then HA was coated using ion beam-assisted deposition (IBAD). HA coating was crystallized by heat-treated at different temperature (100℃, 300℃, 500℃, 800℃). According to the temperature, disks were divided into four groups (HA100, HA300, HA500, HA800). With the temperature, crystallinity of the HA coating was different. Anodized disks were used as control group. The physical properties of the disk surface were evaluated by surface roughness tests, XRD tests and SEM. The effect of the crystallinity of HA coating on HOS cells was studied in proliferation and differentiation. HOS cells were cultured on the disks and evaluated after 1, 3, 5, and 7 days. Growth and differentiation kinetics were subsequently investigated by evaluating cell proliferation and alkaline phosphatase activity.
Results: Regardless of the heat-treated temperature, there is no difference on the surface roughness. Crystallinity of the HA was appeared in the groups of HA500, HA800. HOS cells proliferation, ALP activity were higher in HA500 and HA800 group than HA100 and HA300.
Conclusion: Within the results of this limited study, heat treatment at 500℃ of HA coating produced by IBAD has shown greater effect on proliferation and differentiation of HOS cells. It is considered that further in vivo study will be necessary.
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KEYWORD
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Cell proliferation, Alkaline phophatase activity
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